Effect of agitation and added cholesterol esterase on bioaccessibility of phytosterols in a standardized in vitro digestion model

There is great interest in reaching a consensus method for vitro digestion of foods. The COST INFOGEST network researchers has developed standardized protocol, which appropriate basic digestibility studies, but may be insufficient more complex such as micronutrient bioaccessibility. In the present study, bioaccessibility phytosterols (PS) was evaluated using and modified versions protocol. protocol used without modification 2% solutions either free (FPS) or esterified (EPS) soybean oil. effect two variables have yet to explored subsequently evaluated. Those are: (1) changing mode agitation from orbital shaking head over heels (HOH) rotation (2) adding pancreatic cholesterol esterase (CE) during intestinal phase. Bioaccessibility FPS greater than that EPS, HOH caused further increase not EPS. Addition CE slight, significant improvement though hydrolysis incomplete. Our findings highlight need evaluation especially studies lipid